中国HCV/HIV合并感染者或HIV单一感染者T淋巴细胞表面CD25和CXCR3表达的研究(英文)

Study of expression of CD25 and CXCR3 on T lymphocytes from patients with HCV/HIV co-infection or HIV mono-infection in China

目的探讨T淋巴细胞表面CD25分子和趋化因子受体CXCR3,在丙肝病毒(HCV)单一感染,艾滋病病毒(HIV)单一感染和HCV/HIV合并感染过程中的表达及意义。方法分离HCV感染组(n=21),HIV感染组(n=14),HCV/HIV感染组(n=28)及正常对照组(n=30)人外周血单个核细胞。采用流式细胞术,计数CD4+和CD8+T淋巴细胞数,检测外周血CD4+CD25+T细胞百分含量和CD4+和CD8+T淋巴细胞表面CXCR3的表达水平。结果CD4+T细胞表面CD25的表达在HCV感染组轻度升高,而在HIV感染组及HCV/HIV合并感染组CD25的表达显著降低(P<0.001)。HIV感染组及HCV/HIV合并感染组CD4+T细胞表面CXCR3表达显著降低(P<0.001),CD8+T细胞表面CXCR3表达显著升高(P<0.001);HCV感染组CD4+及CD8+T细胞表面CXCR3表达轻度升高,但差异不显著。结论结果提示中国病毒感染者外周血T淋巴细胞表面CD25和CXCR3分子表达水平和机体免疫调节功能受到HIV感染的影响。

[Objective] To investigate expression and significance of CD25 and CXCR3 on T lymphocytes from peripheral blood mononuclear cells （PBMC） in HCV mono-infection, HIV mono-infection and HCV/HIV co-infection. [Methods] PBMC were isolated from patients with HCV infection （n=21）, HIV infection （n=14）, HCV/HIV coinfection （n=28） and normal control （n=30）. The CD4 and CD8 absolute count, proportion of CD25^＋CD4^＋ T cells, and expression of CXCR3 on CD4^＋ and CD8^＋ T cells from PBMC were analyzed by flow cytometry. [Results] A lower proportion of CD4^＋CD25^＋ T lymphocytes were found in HIV infection and HCV/HIV co-infection （P 〈0.001）, while slight but not significant increased proportion of CD4^＋CD25^＋T lymphocytes were detected in HCV infection. The expression of CXCR3 on CD4^＋T cells from PBMCs decreased significantly, but the expression of CXCR3 on CD8^＋T cells increased significantly in HIV and HCV/HIV co-infection （P 〈0.001）. The expression of CXCR3 on CD4^＋T cells as well as CD8^＋T cells from PBMCs increased but not significantly in HCV infection. [Conclusion] Results suggested that the expression of CD25 and CXCR3 on T lymphocytes from PBMC were altered and immune regulatory function would be effected in HIV infection in China.