旋毛虫感染小白鼠实验研究

A Study on the Model of Small White Mice Infected with Trichinella spiralis

目的:建立小白鼠的旋毛虫病模型,观察研究小白鼠经口感染旋毛虫的合适感染量。方法:将小白鼠分成7组,每只分别感染100,150,200,250,300,350,400个旋毛虫囊包,饲养8周,剖杀,取膈肌特定部位的肌肉,用电子天平称重,然后显微镜下记录囊包数。结果:感染100-400个旋毛虫囊包各组检出旋毛虫囊包数(个/克)依次为:6582±4010,9258±4038,9760±3123,9095±5050,13038±9946,9185±3983,8246±3796,其中,感染300,350,400三组各死亡小鼠一只。结论:小鼠经口感染旋毛虫的合适的感染量为每只小鼠感染150-250个囊包,感染量更高易引起小鼠死亡。

Objective：To investigate and observe the reasonable quantity of encysted larvae of Trichinella spiralis for small white mouse infection. Methods： The small white mice were divided into several groups, and infected with 100, 150,200, 250, 300, 350, 400 encysted larvae respectively. After being reared for 8 weeks, the diaphragm of each mouse in different groups was collected and the number of the cysts in diaphragm was recorded orderly. Results： The number of the cysts per gram of diaphragm muscle in the 7 groups were orderly recorded as follows： 6582 ± 4010,9258± 4038,9760 ±3123,9095 ±5050,13038± 9946,9185± 3983,8246 ± 3796. In addition, dead mice were found in the later three groups. Conclusions： It suggested that 150--250 encysted larvae per mouse should be adopted for mall white mouse infecting with T. spiralis.