多巴胺D_1受体基因-48A/G多态性与妊娠高血压疾病的相关性研究

Relationship between the dopamine D_1 receptor gene polymorphism and the hypertensive disorder complicating pregnancy

目的探讨多巴胺D1受体(DRD1)基因-48A/G多态性与妊娠高血压疾病(HDCP)的关联性。方法运用限制性片段长度多态性—聚合酶链反应分析DRD1基因基因型AA、AG和GG在正常和HDCP孕妇组的分布。结果等位基因A、G在正常孕妇组和HDCP患者的分布频率分别为90.9%、9.1%和72.6%、27.4%,两组基因型频率和等位基因频率差异有统计学意义(P<0.01)。结论在中国汉族人群中,DRD1基因多态性与HDCP相关,但其基因型分布与病情严重程度无相关性。

[Objective] To discuss the correlation between the dopamine D1 receptor （DRD1） gene polymorphism and the hypertensive disorder complicating pregnancy. [Methods] A restriction fragment length polymorphism assay was developed to detect genotypes AA, AG and GG of the human DRD1 gene and their distribution in normal pregnancy and hypertensive disorder complicating pregnancy patients. [Results] The frequencies of the A and G alleles were 89.8% and 10. 2% for the healthy subjects and 72. 5% and 27. 5% for the HDCP group, respectively. Significant differences in the genotype distribution （P〈0. 01） and allelic frequencies （P〈0. 01） between the two groups were observed. [Conclusion] The DRD1 gene polymorphism relates significantly with hypertensive disorder complicating pregnancy.