蛋氨酸亚铁对贫血大鼠补铁效果研究

Effect of Ferrous Methionine(Fe-Met) Supplementation on Biological Utilization of Iron of Anaemic Rats and its Mechanism

选用断奶SD大鼠（体重65g）36只，随机分成3组，天然基础日粮（无铁添加）饲喂15d进行铁耗竭，然后在基础日粮中分别添加FeSO4（I）、FeSO4＋Fe-Met（1：1）、Fe-Met 35mg／kg（以铁计），于补铁后4、7d测定动物生长、血红蛋白、肝脏铁含量及血清抗氧化酶活性指标，以研究不同铁源对大鼠早期生长、氧化代谢和铁利用的影响。结果表明，各组血红蛋白水平在补铁后4d，分别显著提高40．69％、45．02％和50．79％，总铁结合力下降；添加Fe-Met能更迅速提高肝脏铁含量；补铁后1d，Fe-Met组血浆GSH-PX和过氧化氢酶活性略高于FeSO4组，Fe-Met组血浆总抗氧化能力、GSH含量在补铁后1～7d均高于FeSO4组（P〉0．05）；Fe-Met能有效促进动物生长．补铁后7d。Ⅱ组日增重3倍于FeSO4组。试验表明，Fe-Met在促进大鼠生长、肝脏铁贮方面效果显著，并利于早期血红蛋白恢复。在缺铁状态下。无机铁和有机铁混合使用可以相互协调、补充，有利于体内铁状况恢复。

The experiment was conducted to investigate the effect of ferrous sulfate （FeSO4） and/or ferrous methionine （Fe-Met） supplementation on growth, biological value of iron, and serum anti-oxidation index of anaemic rats.36 SD rats（65 g weight） were fed with basal diet（0 Fe added） for 15 days. Then the rats were randomly divided into three treatments and fed with the diets supplied with FeSO4（Ⅰ）, FeSO4 ＋ Fe-Met（1 ： 1） （Ⅱ） and Fe-Met （ Ⅲ） at 35 mg/kg iron respectively for 7 days. The weight gains, blood hemoglobin（Hb）, serum total iron-binding capacity（STBC） and the content of iron in liver of rats were measured after 4 and 7 days respectively. The results showed that the content of Hb was increased 40.69%, 45.02% and 50.79% after 4th day in group Ⅰ, Ⅱ and Ⅲ, respectively. Compared with group Ⅰ,the content of iron in liver was about 19.86% and 11.4% higher in groupⅡ after the supplementation of iron for 4 and 7 days, respectively. The activities of serum GSH-PX and CAT, the content of GSH and total antioxidation capability in serum were also higher in group Ⅱ and Ⅲ than those of group Ⅰ after the supplementation of iron for 1 days. Fe-Met could notably promote the rats weight, which were triple times in groupⅡ than that of the FeSO4 group in 7 th days of iron supplement It was concluded that Fe-Met could rapidly improve the biological utilization of iron.