金属离子对悬浮培养霍山石斛类原球茎合成活性多糖的影响

Effect of Metal Ion on the Polysaccharide Synthesis in Protocorm-like Body of Dendrobium huoshanense in Suspension Culture

采用单因素试验和正交试验,考察了Mg2+、Ca2+、Fe2+、Mn2+和Zn2+5种金属离子对悬浮培养霍山石斛类原球茎合成活性多糖的影响。结果表明:1.5 mmol/L Mg2+4、.5 mmol/L Ca2+0、.1 mmol/L Fe2+0、.5 mmol/L Mn2+和0.06 mmol/L Zn2+的组合最有利于类原球茎多糖的合成,在此组合下多糖产量达最高(5.87 g/L),比优化前提高2.49倍,比标准MS培养基提高31倍,且5种离子对类原球茎合成多糖影响的主次顺序为Mg2+>Ca2+>Mn2+>Fe2+>Zn2+。

This research is concerned with the effect of Mg^2＋ , Ca^2＋ , Fe^2＋ , Mn^2＋ and Zn^2＋ on polysaccharide synthesis in Pretoconn-like body of Dendrobium husoshanense in suspension culture through the orthogonal matrix method with one factor-at-a-time. The results showed that the optimal combination for polysaccharide accumulation in protocorm-like body was 1.5 mmol/L Mg^2＋ ,4.5 mmol/L Ca^2＋ ,0.1 mmol/L Fe^2＋ ,0.5 mmol/L Mn^2＋ and 0.06 mmol/L Zn^2＋. The peak of the polysaccharide production（5.87 g/L） was obtained in the culture medium with the same ion combination mentioned above, which was increased 2.49-fold than that of the former experiment and was increased 31-fold than that obtained in standard MS. Furthermore, the responsible order of metal ion in the polysaccharide synthesis was Mg^2＋ 〉 Ca^2＋ 〉 Mn^2＋ 〉 Fe^2＋ 〉 Zn^2＋.