DNA微阵列检测异氟醚麻醉的基因表达

CDNA microarray analysis of the differential gene expression by isoflurane anesthesia

目的用DNA微阵列筛选异氟醚麻醉的表达基因。方法12只雄性Wister大鼠,分为2%异氟醚组(A组)和对照组(B组)。A组用2%异氟醚麻醉1h,B组直接处死后取脑组织。提取RNA,荧光标记,用有4096个位点的BiostarR40s基因芯片,检测差异表达的基因。结果发现了26个差异表达的基因,其中17个基因下调,9个基因上调。结论基因芯片检测出2%异氟醚麻醉1h有26个差异表达基因。

Objective To investigate the gene expression profiles from rats under isoflurane anesthesia with DNA microarrays. Methods Six-week-old male Wister rats （n= 12） were anesthetized with isoflurane （2% air mixture gas）. At 1 h after anesthesia, rats were sacrificed to obtain the brain. RNA was extracted. CDNA probe labeled with fluorescence dye was made from poly A ＋ RNA, hybridized with the BiostarR-40s rat microarray slide containing 4 096 genes. Results Preliminary analyses showed multiple genes were regulated in response to isoflurane treatment. There were 26 genes changed expression,of which, the expression levels of 17 genes decreased after isoflurane anesthesia and 9 genes upregulated. Conclusion By analyzing expression profiles obtained from rats under isoflurane anesthesia, we have shown that isoflurane anesthesia affected the expression of a small number of genes.