籼粳杂交F_1高效花药培养技术体系的建立

Establishment of technology system of F_1 anther culture from japonica/indica hybrid rice

以黑壳子粳／苏御糯、铁杆青／苏御糯和薄稻／苏御糯3个籼粳杂交F1及其亲本和粳稻品种武运粳8号为试验材料，在Ms基本培养基中，加入不同浓度的植物激素类物质和有机营养物，建立籼粳杂交F1高效花药培养技术体系。结果表明：Ms＋2mg·L^-1 2，4-D＋1mg·L^-1NAA＋0．2mg·L^-1KT为最佳诱导培养基，籼粳杂交F1花约愈伤组织诱导率达4．1％～4．5％；在该培养基中添加水解酪蛋白或酵母提取物，能明显提高诱导率，增幅达20．5％-27．3％；Ms＋2．5mg·L^-1 6-BA＋0．5mg·L^-1 KT为最佳分化培养基，籼粳杂交F1的愈伤组织绿苗分化率达14．3％～25．0％。

The anthers of F1 progenies from three crosses between japonica and indica rice varieties Heikezijing/Suyunuo, Tieganqing/Suyunuo, Bodao/Suyunuo and their parents, and one japonica variety Wuyunjing 8 were used to induce the calli in the Ms medium supplemented with different concentrations and combinations of plant hormones and organic nutriments. A highly efficient system of anther culture was established for F1 progenies between japonica and indiea rice varieties. The results showed that the highest rate of callus induction （4.1%-4.5% for japoniea/indiea F1 hybrid ） was obtained in the Ms medium supplemented with 2 mg·L^-1 2,4-D, 1 mg·L^-1 NAA and 0. 2 mg·L^-1 KT. The induction rate could be further increased up to 20. 5%-27.3% in this medium added with hydrolyzed casein and yeast extract, it could be obtained the 14.3%-25.0% of plant regeneration rate （japonica/indica F1） in the Ms medium added with6-BA （2.5mg·L^-1） and KT （0.5 mg·L^-1）.