摘要
以一株具有较高纤维素酶活性的野生菌株-灰绿曲霉XC9为出发菌株,经过紫外线、氯化锂、甲基磺酸乙酯(EMS)等物理化学诱变剂多重诱变处理,获得了抗葡萄糖阻遏的突变株E2-26、E5-65、U6-31和EU7-22,其CMC酶活与出发菌株相比分别提高至1.7、1.4、1.3、2.0倍.突变株经PDA斜面接种传代5次后产酶性能仍然保持稳定.变株的菌丝、孢子颜色也发生了较大的改变.对突变株EU7-22的形态结构进行了观察,并与出发菌株XC9作了发酵性状的比较,发现其产酶性能有了明显提高.
Cellulase producing strain, Aspergillus glaucus XC9, was selected as the initial swain for mutation. Four mutant strains （E2-26, E5-65, U6-31, EU7-22） were obtained after it was treated multiplicatively with UV-ray, ethyl methane sulphonate （EMS） and LiCl. The CMC activity of mutants increased to 1.7, 1.4, 1.3 and 2.0 times comparing with the initial strain respectively. The spore colours of mutants also varied. The morphological and fermentative properties of mutant strain EU7-22 were studied in this paper. The producing capability of cellulase of mutant swain was remarkably enhanced.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第B05期23-26,共4页
Journal of Xiamen University：Natural Science
基金
国家"863"计划项目(2001AA515040)
福建省重点科技项目(2005I016)
福建省青年科技人才创新项目(2005J003)
厦门市科技项目(3502Z20041070)资助
关键词
灰绿曲霉
纤维素酶
诱变育种
抗葡萄糖阻遏
Aspergillus glaucus
cellulase
mutation breeding
glucose repression-resistance
