南方菟丝子18S rRNA基因片段的克隆与序列分析

Cloning and Sequence Analysis of 18S Ribosomal RNA Gene Fragment from Cuscuta australis R.Br.

根据拟南芥光敏色素B基因序列设计引物,RT-PCR扩增南方菟丝子同一基因相应片段,扩增使用了TD PCR技术,同时获得3个特异基因片段,对长约300 bp的片段克隆后进行序列分析,显示该片段与沼泽菟丝子和拟南芥18SrRNA基因相应片段的一致性分别为98.9%和97%,结果表明,该片段为南方菟丝子18S rRNA基因片段.

The primer was designed according to two conserved regions of Phytochrome. B of Arabidopsis thaliana to amplify the same gene fragment from Cuscuta australis R. Br. cDNA by Touch Down PCR. Three gene fragments were obtained. One of the fragments about 300 bp was cloned and sequenced, and the sequence analysis result showed the nucleotide sequence of this gene fragment was consistent with the sequence of the correspondent segments of 18S ribosomal RNA gene in Cuscuta gronovii Willd and Arabidopsis thaliana at the uniformity rates of 98.9% and 97% respectively. The result reveals that it is 18S rRNA gene fragment of Cuscuta australis.