摘要
角质细胞生长因子(Keratinocyte growth factor,KGF)在组织的损伤修复中起着重要的作用.在植物里面表达高活性的KGF具有成本低、易开发的优点.本实验将人角质细胞生长因子基因经农杆菌介导转入水稻中,获得110株再生植株.对再生植株进行GUS检测发现有44.7%植株呈阳性,PCR检测的结果也显示有52.6%的植株为阳性植株.
Keratinocyte growth factor （KGF） plays an important role in the wound repair. It is economical to express KGF in plant. In this study, we introduced human KGF gene into rice mediated by Agrobacterium. 110 independent seedlings regenerated from transformants have been obainted. The positive ratio of the seedling is 44.7% by GUS detection and 52.6% by PCR detection.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第B05期70-72,共3页
Journal of Xiamen University：Natural Science
关键词
角质细胞生长因子
遗传转化
基因表达
keratinocyte growth factor
transformation
gene expression
