车前子对抗高脂血症大鼠脂质过氧化作用(英文)

Effect of plantain seed on the lipid peroxidation in rats with hyperlipidemia

背景:近年研究发现,车前草种子外壳是很好的可溶性膳食纤维源,可用来添加到食品中,调整胆固醇含量。目的:观察车前子对高脂血症大鼠血脂及其体内脂质过氧化作用的干预结果。设计:完全随机分组设计,对照动物实验。单位:河北省新药药理毒理实验室。材料:①选用健康一级SD大鼠24只,鼠龄60～70d,体质量(210±22)g,雌雄不拘。②基础饲料由河北省实验动物中心配制,各成分质量分数:面粉0.25,麸皮0.1,玉米面0.22,豆饼0.22,鱼粉0.08,骨粉0.02,草粉0.05,食盐0.01,酵母粉0.02,葵花子0.03。高脂饲料由河北省实验动物中心配制,各成分质量分数:基础饲料0.9,胆固醇0.015,猪油0.08,猪胆盐0.003。③测定血脂试剂盒由保定长城临床试剂公司出品;测超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活力和丙二醛水平试剂盒由南京建成生物工程研究所提供。方法:实验于2004-06/12在河北省新药药理毒理研究实验室完成。①随机将24只大鼠分为3组:正常对照组,模型组,车前子组,每组8只。正常对照组:进食基础饲料。模型组:进食高脂饲料。车前子组:进食高脂饲料+车前子15g/kg,普通饮水。实验动物分笼饲养,每只摄食25g/d,自由饮水,实验周期为12周。②实验结束时,麻醉大鼠,采用相应试剂盒测定血清三酰甘油、总胆固醇、高密度脂蛋白胆固醇水平;采用相应试剂盒测血清超氧化物歧化酶、过氧化氢酶活力和丙二醛水平,心肌组织超氧化物歧化酶活力及丙二醛含量,肝组织过氧化氢酶和谷胱甘肽过氧化物酶活力。③两组间计量资料差异比较采用t检验。主要观察指标:造模12周后,各组大鼠血脂水平及机体抗氧化能力比较。结果:大鼠24只均进入结果分析。①造模12周后,模型组大鼠血清和心肌组织超氧化物歧化酶活力明显低于正常对照组和车前子组(P<0.05),血清丙二醛水平和心肌丙二醛含量均明显高于正常对照组和车前子组(P<0.05)。②造模12周后,模型组大鼠血清和心肌组织过氧化氢酶和肝组织谷胱甘肽过氧化物酶活力均明显低于正常对照组和车前子组(P<0.05)。③造模12周后,模型组大鼠血清总胆固醇和三酰甘油水平明显高于正常对照组和车前子组(P<0.05),血清高密度脂蛋白胆固醇水平和高密度脂蛋白胆固醇/总胆固醇明显低于正常对照组和车前子组(P<0.05)。结论:提示15g/kg剂量车前子可明显降低高脂血症大鼠血脂,增加机体抗氧化能力。

BACKGROUND： Recently, it is investigated that shell of plantain seed is a soluble dietary fiber which can be added into foods to regulate content of cholesterol. OBJECTIVE： To investigate the interventional effect of plantain seed on lipid and its lipid peroxidation in rats with hyperlipidemia. DESIGN： Completely randomized grouping design and controlled animal study. SETTING： Laboratory of Pharmacology and Toxicology for New Drug in Hebei Province. MATERIALS： ① A total of 24 healthy SD rats, of grade Ⅰ, aged 60-70 days, weighting （210±22） g, of either gender, were selected in this study. ② Basic feed was provided by Experimental Animal Center in Hebei Province, and the fractional mass of each component was mentioned as following： flour 0.25, bran 0.1, corn dust 0.22, bean cake 0.22, fish dust 0.02, bone dust 0.02, grass dust 0.05, salt 0.01, yeast dust 0.02, and sunflower seed 0.03. High fat feed was provided by Experimental Animal Center in Hebei Province, and the fractional mass of each component was mentioned as following： basic 0.9, cholesterol 0.015, lard 0.08, and hyocholic salt 0.003. ③ Lipid kit was provided by Baoding Changcheng Clinical Reagent Company, and kits of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-Px） and malondialdehyde （MDA） were provided by Nanjing Jiancheng Bioengineering Institute. METHODS： The experiment was completed at the Laboratory of Pharmacology and Toxicology for New Drug in Hebei Province from June to December 2004. ① All 24 rats were randomly divided into 3 groups： normal control group, mode/group and plantain seed group with 8 in each group. Rats in the normal control group were fed with basic feed. Rats in plantain seed group were fed with high fat feed ＋ 15 g/kg plantain seed and drank routinely. Experimental rats were fed in cages, respectively. Each one was fed with 25 g/d food and drunk freely. The experimental cycle was 12 weeks. ② At the end of experiment, rats were anesthetized to assayed levels of serum triacylglycerol （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C）, serum SOD and MDA, activities of CAT and SOD in myocardial tissue, content of MDA, and activities of CAT and GSH-Px in hepatic tissue with related kits. ③ Measurement data were compared between each two group with t test. MAIN OUTCOME MEASURES：Comparison of serum lipid level and anti-oxidation among groups at 12 weeks after modeling. RESULTS： All 24 rats were involved in the final analysis. ①At 12 weeks after modeling, activities of SOD in serum and myocardial tissue were lower in model group than those in normal control group and plantain seed group （P 〈 0.05）, but levels of MDA in serum and myocardial tissue were higher in model group than those in normal control group and plantain seed group （P 〈 0.05）. ② At 12,weeks after modeling, activities of CAT and GSH-Px in serum and myocardial tissue were lower in model group than those in normal control group and plantain seed group （P 〈 0.05）. ③ At 12 weeks after modeling, levels of TC and TG in serum were higher in model group than those in normal control group and plantain seed group （P 〈 0.05）, but level of HDL-C and ratio between HDL-C and TC in serum were lower in model group than those in normal control group and plantain seed group （P 〈 0.05）. CONCLUSION： Plantain seed at dosage of 15 g/kg can decrease content of lipid and strengthen antl-oxidation of economy in rats with byperlipidemia.