海南红树林的微生物生态分布及细胞毒活性评价

Isolation and Cytotoxic Activity Evaluation of Microorganisms from Mangrove in Hainan

从海南清澜港18种红树和4种半红树植物的土壤、根、叶和果中分离微生物,利用目测法对微生物发酵液进行小鼠黑色素瘤B16细胞毒活性筛选。结果表明,从银叶树(Heritieralittoralis)、黄槿(Hibicustilisaceus)和杯萼海桑(Sonneratiaalba)中分离筛选得到的总菌数和活性菌株高于其他被研究的红树品种。在所采集的土壤、根、叶和果的样品中,红树林土壤中含有最多的强细胞毒活性菌株,其次是根,叶和果中的数量最低。不同样品类型以及细菌、放线菌、真菌等不同微生物类型与细胞毒活性之间有显著相关性,分别从土壤样品类型和放线菌微生物类型获得最高比例的活性菌。

Microorganisms were isolated from the samples of soil, roots, leaves and fruits of 18 species of real mangroves and 4 species of semi-mangrove collected in Qinglan Port, Hainan. Cytotoxic activity of the culture supernatant of the isolates against murine melanoma ceils B16 were evaluated by observing the growth inhibition or killing of the tumour cells under microscope. Number of total microorganisms isolated, and cytotoxic active strains were found higher from mangrove species of Heritiera littoralis Hibiscus tiliaceus and Sonneratia alba than the other mangrove species under observation. Among the samples of soil, root, leaf and fruit collected, the number of total and active strains isolated from the soil sample was the largest, followed by the root, and the lowest are from the leaf and fruit samples. Statistical analysis showed that there was a significant relation between the cytotoxic activity of the isolated strains and the sample types as well as different microorganism types such as bacteria, actinomycetes and fungi. The soil sample type and the microbe type of actinomycetes produced the highest percentage of active strains respectively.