逆境下水稻(Dryza sativa L.)rHsp90基因的克隆及功能分析

Cloning and Characterization of the rHsp90 Gene in Rice (Oryza sativa. L) under Environmental Stress

本研究中,利用差异显示法,在碳酸盐逆境下,从水稻(OryzasativaL.)根的cDNA文库中克隆得到了水稻热激蛋白90基因(rHsp90,GenBankAccessionNo.AB037681)。Northern杂交结果表明,在水稻根中,rHsp90基因的转录水平在包括盐(NaCl,NaHCO3和Na2CO3),PEG,高pH(pH8.0和pH11.0)以及热激(50℃)逆境下,都受到了不同程度的诱导。同时,适量表达rHsp90基因的酵母(Saccharomycescerevisiae)在各种盐逆境以及热激逆境中,生长状态要好于对照。对转水稻rHsp90基因烟草的抗盐(NaCl,NaHCO3)分析表明,转基因烟草的生长势要好于野生型烟草。通过以上研究表明,水稻rHsp90基因与逆境之间具有一定的应答关系,并在植物适应环境逆境过程中起着重要的作用。

In this study, the rice （Oryza sativa L） heat shock protein 90 kD gene （rHsp90, G-enBank Accession No. AB037681） was cloned by screening rice root cDNA library constructed from the plants grown under carbonate stress with the method of differential display. Northern blot analysis showed the result that the accumulation of rHsp90 mRNA was observed in rice root under salts （NaCl, NaHCO3 and Na2CO3）, PEG, high pH conditions （pH8.0 and pill 1.0） and high temperature （50℃） conditions. Furthermore, the yeast （Saccharomyces cerevisiae） suitable expressing rHsp90 exhibited tolerance to salt and heat shock stress. Meanwhile, the tobacco seedlings over-expressing rHsp90 showed better growth performance than untransformed control seedlings under salt （NaCl, NaHCO3） stress. These results suggested that the expression of rHsp90 gene was enhanced in response to several abiotic stresses, and it played an important role in plant adaptation to multiple environmental stresses.