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利用SSR标记定位粳稻云引抗稻瘟病基因 被引量:12

Mapping Blast-resistance Genes of Japanica Rice Yunyin by SSR Markers
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摘要 稻瘟病是由子囊菌Magnaporthegrisea(Hebert)Barr[无性世代为Pyriculariagrisea(cooke)Sacc.]引起的广泛发生在我国南北稻区及世界各稻区的主要水稻病害之一,严重阻碍水稻高产与稳产。挖掘和利用广谱稻瘟病抗源,通过基因累加手段将不同抗稻瘟病基因聚集于一体,可延长抗稻瘟病品种应用年限。本研究应用6个稻瘟病菌系四川-1、四川-4、四川-9、四川-31、四川-39和四川-41,对广谱抗性粳稻品种云引F2群体进行田间注射接种,结果表明云引F2群体对所用的6个稻瘟病菌系均表现为3(抗病):1(感病)的分离比例,说明这些抗性均为单显性基因控制。本研究利用Mapmaker3.0/QTL将云引对四川-1菌系的抗稻瘟病基因初步定位在水稻的第11染色体上,云引对四川-4、四川-9、四川-31、四川-39和四川-41的抗稻瘟病基因初步定位在第2染色体上。 Rice blast disease, caused by fungal pathogen Pyricularia grisea Sate., is one of the most serious diseases of rice (Oryza sativa L.) in north-south of China and worldwide. It is one of the major factors that obstacle high yields and stable production in rice. Excavating and utilization of wide blast-resistance rescources, pyramiding different blast-resistance genes into cultivated varieties of rice can prolong blast-resistance times. 6 rice blast disease races, i.e. Sichuan-l.Sichuan-4.Sichuan-9.Sichuan-31.Sichuan-39 and Sichuan-41, were injected into the F2 population of the japan/ca rice Yunyin in the field. The results indicated that blast-resistance genes of japanica variety of Yunyin to the 6 races is controlled by single dominant gene respectively. Moreover, using software Mapmaker 3.0/QTL, the blast-resistant genes to Sichuan-1 was primarily mapped in chromosome 11, the blast-resistant genes to Sichuan-4. Sichuan-9. Sichuan-31 .Sichuan-39 and Sichuan-41 were all primarily mapped in chromosome 2.
出处 《分子植物育种》 CAS CSCD 2006年第3期359-364,共6页 Molecular Plant Breeding
基金 福建省科技厅重大项目“优质、高产、抗病超级杂交水稻新组合选育”(20022001) 科技部“杂交稻育种创新与应用”(2001BA507A10-09)项目资助.
关键词 粳稻 云引 抗稻瘟病基因 遗传 基因定位 Japanica flee, Yunyin, Blast-resistance genes, Inheritance, Mapping
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参考文献36

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