T cell immune response is correlated with fibrosis and inflammatory activity in hepatitis B cirrhotics

瞄准：在 interferon-gamma (IFN-gamma ) 之中探索关系活动，纤维发生， T 房间免疫者回答和肝的煽动性的活动。方法：从 43 个肝炎 B 肝脏硬化症的病人( LC )和 19 健康控制( NC )的一个总数的外部血样品被收集测量他们 IFN-gamma 的浆液层次， interleukin-2 ( IL-2 )，可溶的 interleukin-2 受体( sIL-2R )， interleukin-10 ( IL-10 )和包括透明质酸的纤维变性的三个血清学的标记(哈)，职业人员骨胶原类型 III 肽(果仁)，和类型 IV 骨胶原用双抗体三明治 ELISA 被测量。另外，浆液总数胆红素(TB ) 和丙氨酸 aminotransferase (中高音) 被平淡的措施测量。结果：在有活跃肝硬化(ALC ) 的病人的纤维变性的血清学的标记的集中比在静止的肝肝硬化(SLC ) 的那些显著地高或 NC 组织。在 HBeAg 积极的病人的血清学的标记的层次比在 HBeAg 否定的病人的那些显著地高。在 SLC 和 ALC 病人，一道否定直线相关在 IFN-gamma 层次和纤维变性的血清学的标记之间被发现。在 ALC 组的 IFN-gamma 和 IL-2 层次比在 SLC 和 NC 组的那些显著地高，但是统计差别不在后者之间是重要的二。相反，在 SLC 组的 IL-10 层次在 NC 组比那显著地高，但是没有有效差量在 SLC 和 ALC 组之间被发现。sIL-2R 水平在所有这些组逐渐地被提高，并且差别是重要的。积极直线相关在 IFN-gamma 活动和中高音层次之间被看见(r = 0.339， P 【 0.05 ) ，并且 IL-2 活动和 TB 层次(r = 0.517， P 【 0.05 ) 。sIL-2R 表示断然与中高音和 TB 层次被相关(r = 0.324， 0.455， P 【 0.05 ) ，而在 IL-10 之间没有统计上重要的关联，表示和浆液中高音和 TB 铺平(r =-0.102,-0.093, P 】 0.05 ) 。最后，在 IFN-gamma 和 IL-2 层次之间有积极关联。结论：T 房间免疫者回答与纤维变性和肝的煽动性的活动被相关并且可以在肝肝硬化起一个重要作用。

AIM： TO explore the relationship among interferon-γ （IFN-γ） activity, fibrogenesis, T cell immune responses and hepatic inflammatory activity. METHODS： Peripheral blood samples from a total of 43 hepatitis B cirrhotic patients （LC） and 19 healthy controls （NC） were collected to measure their serum levels of IFN-γ, interleukin-2 （IL-2）, soluble interleukin-2 receptor （sIL-2R）, interleukin-10 （IL-10） and three serological markers of fibrosis including hyaluronic acid （HA）, procollagen type III peptide （PIIIP）, and type iV collagen were measured using a double antibody sandwich ELISA. Also, serum total bilirubin （TB） and alanine aminotransferase （ALT） were measured by routine measures. RESULTS： The concentrations of serological markers of fibrosis in patients with active cirrhosis （ALC） were significantly higher than those in stationary liver cirrhosis （SLC） or NC groups. The levels of serological markers in HBeAg-positive patients were significantly higher than those in HBeAg-negative patients. In SLC and ALC patients, a negative linear correlation was found between IFN-γ levels and the serological markers of fibrosis. IFN-γ and IL-2 levels in the ALC group were significantly higher than those in the SLC and NC groups, but the statistical difference was not significant between the latter two. In contrast, IL-10 levels in the SLC group were significantly higher than that in the NC group, but no significant difference was found between SLC and ALC groups. The sIL-2R level was elevated gradually in all these groups, and the differences were significant. Positive linear correlations were seen between IFN-γ activity and ALT levels （r = 0.339, P 〈 0.05）, and IL-2 activity and TB levels （r = 0.517, P 〈 0.05）. sIL-2R expression was positively correlated with both ALT and TB levels （r = 0.324, 0.455, P 〈 0.05）, whereas there was no statistically significant correlation between IL-10 expression and serum ALT and TB levels （r = -0.102, -0.093, P 〉 0.05）. Finally, there was a positive correlation between IFN-γ and IL-2 levels. CONCLUSION： T cell immune responses are correlated with fibrosis and hepatic inflammatory activity and may play an important role in liver cirrhosis.