大鼠脑内成分对体外培养的成骨细胞增殖、分化和矿化的影响

The effect of tissue fluid extracted from rat brains on osteoblast in vitro

目的研究大鼠脑组织固有成分对体外培养的成骨细胞增殖、分化和矿化的影响,探讨合并脑外伤骨折愈合加快的可能原因。方法提取大鼠全脑、灰质、白质、垂体、肌肉、肝脏、大鼠血清等,制成匀浆提取液,体外培养大鼠成骨细胞,与空白培养液作对照观察大鼠各组织提取液对成骨细胞增殖、分化和矿化的作用。结果对成骨细胞增殖的影响:脑内灰质和白质成分较其他提取液显著促进成骨细胞增殖(P<0.01),其中灰质成分最为明显;对成骨细胞分化的影响:灰质,白质,血清成分对成骨细胞ALP活性的影响明显较其它各组高(P<0.01),其中灰质成分为最高(P<0.01);对成骨细胞矿化的影响:肌肉组轻度抑制成骨细胞矿化结节形成(P<0.05),其他各组无显著性差别。结论大鼠脑组织中灰质和白质能显著促进成骨细胞增殖和分化,尤以灰质为最强,但并不能明显促进成骨细胞矿化。脑外伤后脑组织内固有成分释放入血可能和合并脑外伤的骨折愈合加速有一定关系。

Purpose To study the effect of tissue fluid extracted from the rat brain inherent components on osteoblast proliferation, differentiation and mineralization in vitro ,and to analyze the possible cause of enhanced fracture healing combined with brain injury. Methods The tissue fluid was extracted from the rat total brain, white matter, gray matter, pituitary, liver and muscle. The rat osteoblast was cultured in the tissue fluids in vitro, then the effect was observed on rat osteoblast proliferation, differentiation, and mineralization comparing with the blank culture media. Results The effect on osteoblast proliferation： the gray matter group and the white matter group had more proliferation stimulated effect comparing with the control group（P〈0.01）, and the gray matter group had a most proliferation stimulated effect. The effect on osteoblast differentiation： the gray matter group, the white matter group and the serum group had more proliferation timulated effect comparing with the control group（P〈0. 01）, and the osteoblast ALP activity in the gray matter group were the highest in all groups（P〈0.01）. The effect on osteoblast mineralization： the muscle group had a relative significant inhibitation in the osteoblast mineralization（P〈0.05）. Conclusions The gray matter and white matter of rat brain tissue could significantly stimulate the osteoblast proliferation and differentiation, the gray matter was especially more powerful, but they couldn＇t stimulate the osteoblast mineralization. It seems that the inherent materials in the brain releasing into the blood after cerebral trauma might have relationship with enhanced fracture healing combined with brain injury.