摘要
对肌动蛋白基因在丝瓜（Ｌｕｆｆａ ｃｙｌｉｎｄｒｉｃａ Ｌ．）和黄瓜（Ｃｕｃｕｍ ｉｓ ｓａｔｉｖｕｍ Ｌ．）各器官的表达进行了研究．Ｎｏｒｔｈｅｒｎ ｂｌｏｔ和Ｄｏｔｂｌｏｔ结果表明，肌动蛋白基因在丝瓜和黄瓜中表现出器官特异性表达． 首先，肌动蛋白基因在丝瓜幼苗发育过程中表现明显的发育阶段特异性． ３０ ｄ 苗茎的ｍ ＲＮＡ 水平为８ ｄ 苗的根、子叶和１５ ｄ 苗的根、下胚轴的４～６ 倍， 同时为开花植株茎和叶片的ｍ ＲＮＡ 水平的１０～１２ 倍． 其次，肌动蛋白基因在黄瓜中表现器官特异性表达，它们倾向于在黄瓜幼嫩果实中（开花后１５
Results of Northern blot and Dot blot analysis indicated that actin genes exhibit organ specific expression in snake gourd (Luffa cylindrica L.) and cucumber (Cucumis sativum L.). Actin genes showed obvious developmental specificity during the development of snake gourd seedlings.mRNA levels in stems of 30 day old seedlings were 4～6 times higher than that of roots and cotyledons of 8 day old seedlings and roots and hypocotyls of 15 day old seedlings,and were even 10～12 times higher than that of stems and leaves of flowering plants.Actin genes also showed organ specific expression in young fruits (15 days after flowering) of cucumber.
基金
国家自然科学基金
关键词
丝瓜
黄瓜
肌动蛋白
肌动蛋白基因
基因表达
Actin
Actin genes
Snake gourd
Cucumber
Organ specific expression
