中国蜜环菌生物种的RAPD分析

PHYLOGENETIC RELATIONSHIPS OF BIOLOGICAL SPECIES OF ARMILLARIA IN CHINA ANALYSED BY RANDOMLY AMPLIFIED POLYMORPHIC DNA

以中国蜜环菌(Armillaria mellea(vahl:Fr.)Kummer)生物种B.生物种E分别与欧洲种A.gallica和A.ostoyae交配,用AP-PCR技术分析了中国五个生物种及欧洲2个种的代表菌株的系统发育关系。根据系统聚类分析的结果,将其分为4个群:生物种B与A.gallica,A与C,D与E,A.ostoyae单独为一群。这与交配试验及RAPD图谱直观分析的结果一致,最小支撑树也支持将中国生物种B鉴定为A.gallica。证明RAPD是研究蜜环菌生物种的进化关系的有用手段。

Chinese biological species B and E were compatible to European species A. gallica and A . ostoyae respectively. Phylogenetic relationships among haploid isolates of five Chinese biological species and two European ones have been analysed by AP - PCR with six primers. Seven isolates were divided into four groups based on the result of hierarchical clustering analysis, i. e. species B and A . gallica, A and C , D and E as well as A . ostoyae. This is conformable to the results of pairing tests and direct observation of RAPD patterns. Results of Minimum Spanning Trees (MST) also suggest that biological species B may be identified as the same as A . gallica. Our study suggests that RAPD is a useful tool for research on evolutionary relationships of biological species of Armillaria,