土人参的组织和单细胞培养及试管苗开花结实

TISSUE AND MONOCELL CULTURE AND IN VITRO FLOWERING AND FRUITING OF TALINUM PANICULATUM

以土人参的花梗、茎和叶片为外植体在ＭＳ培养基上诱导出愈伤组织，诱导率为７５％─９０％。愈伤组织经分化和生根培养再生了完整植株。由组织培养再生苗的幼茎诱导的愈伤组织建立悬浮系。由悬浮系分离的单细胞在２／３ＭＳ液体培养基中振荡培养或振荡培养３周后转入双层培养均再生了愈伤组织，再生率分别为０．２８％和０．４１％。愈伤组织在含有较低浓度６－ＢＡ的培养基上分化出苗。幼苗生长迅速，每３周扩增６．７倍，再生植株移栽到土壤中２－３个月开花结实，并长出粗壮的肉质根。再生小植株在试管中继代２－３个月也可开花结实。本文还讨论了愈伤组织的增殖与继代培养对分化的影响以及６－ＢＡ、ＧＡ３和ＭＥＴ在分化和生长发育中的作用。

Calli were induced with frequencies of 75%- 90% using young pedicels,stems and leaves of Talinum Paniculatum(Jeaq. ) Geartn on MS medium. And then, the whole plants were regenerated from the calli via organogenesis.Suspension lines were established with calli induced from stems by vibration culture. Monocells were isolated from the suspension lines and were cultured in liquid or double-layer MS medium containing 1. 0 or 2. 0 mg/L of 2, 4-D. After 35 days of culture, microcalli were regenerated with frequencies of 0. 28 % in suspension culture and 0.41 % in double-layer culture respectively. The monocell calli differentiated adventitious buds on media containing lower concentration of 6-BA (0. 5 - 0. 7 mg/L). The shoots grew rapidly with as high as 6. 7 of propagation coefficient of three weeks. The regeneration plants were transplanted into soil for further growing and developing. And the mature plants were obtained after 2 -3 months of transplantation. Besides,the regeneration plantlets undergone 2- 3 months of subculture flowered and fruited in culture tubes.In this paper, effects of propagation and stlbculture of callus, 6-BA, GA3 and MET on differentiation,growth and development were discussed.