从玉米胚中大量制备植物钙调素

MASS PREPARATION OF PLANT CALMODULIN FROM MAIZE GERM

从每公斤萌发２４ｈ的玉米胚两酮粉中可提纯得６３ｍｇ的钙调素（ＣａＭ），这是目前从每公斤植物材料中所提纯得ＣａＭ的最高记录。对其理化性质的研究表明，玉米胚ＣａＭ有较高的生物学活性，能较好地激活磷酸二酯酶，其紫外吸收光谱，ＳＤＳ－ＰＡＧＥ电泳行为及氨基酸组成与其它的植物ＣａＭ相似。上述结果表明玉米胚是１个提取植物ＣａＭ的适宜材料。

mg calmodulin was extracted and purified from 1 kg acetone powder of maize germ germinated for 24 h, which is the highest record for the quantity of calmoulin purified from 1 kg plant materials. Our results showed that the calmodulin from maize germ had higher biological activity, could activate cAMP phosphodiesterase within a good range, and its UV absorption spectrum, electrophoretic mobility on SDS-PAGE in the presence or absence of Ca2+ and amino acid composition was similar to those calmodulins from other plant materials. The above-mentioned results indicated that maize germ is a suitable material for the extraction and purification of plant calmodulin.