用显微分光光度术测定不同发育时期蚕豆子叶淀粉质体DNA含量

MICROPHOTOMETRIC DETERMINED OF AMYLOPLASTS DNA CONTENT OF DIFFERENT DEVELOPMENTAL PERIODS IN COTYLEDON CELL OF BROAD BEAN

叙述了用显微光度术在亚细胞水平上测量蚕豆子叶细胞中淀粉质体Ｆｅｕｌｇｅｎ－ＤＮＡ含量，显示了在不同发育时期蚕豆子叶细胞中的淀粉体ＤＮＡ含量的动态变化。随着发育时期的递增，淀粉体ＤＮＡ拷贝数不断地增长。生长中期的淀粉体ＤＮＡ含量平均数为２．１４（任意单位），到了成熟期淀粉体ＤＮＡ平均含量增长到１０．１２（任意单位）。在成熟期，子叶细胞中的淀粉体ＤＮＡ含量比生长中期增长了９．６倍。通过生物统计分析，在亚细胞水平上质体间ＤＮＡ含量上的变量变化显示出了生物学上自然对数正态分布规律。

The Feulgen-DNA contents of amyloplasts in cotyledon cell of broad bean in sub-cellular level have been determined by microphometry and the result showed the dynamic change in different developmental periods. The amyloplasts DNA of copies increase with the developmental stage-The average of amyloplasts DNA content is 2.14(arbitrary unit) in the middle stage of growth. Amyloplasts DNA increase to 10.12 (arbitrary unit) in the mature period of cotyledon cell of broad bean. Amyloplasts DNA content in cotyledon cell of mature period is 9.6 times than that in the middle period of growth.The change of variability DNA contents among plastids showed normal distribution in natural logarithm by biological statistics.