烟草花粉原生质体与叶肉原生质体的融合及培养早期变化

Fusion Between Pollen Protoplasts and Mesophyll Protoplasts of Nicotiana and Early Changes of Culture

用ＰＥＧ—高Ｃａ高ＰＨ法诱导抗卡那霉素的烟草（Ｎｉｃｏｔｉａｎａｔａｂａｃｕｍ）品系Ｎ３６４＋Ｋｍ＋花粉原生质体和黄花烟草（Ｎｉｃｏｔｉａｒｕｓｔｉｃａ）叶肉原生质体融合。幼嫩花粉原生质体和叶肉原生质体之间的融合体培养启动胚胎发生分裂，经卡那霉素筛选后，少数多细胞团存活并形成小愈伤组织。成熟花粉原生质体与叶肉原生质体之间的融合体则仅产生管状结构。这一结果表明，作为融合一方的花粉原生质体的发育时期对融合产物的发育途径有重要影响。

Previous result by SDS PAGE showed that the HMW glutenin subunits of Longmai 15 were 2+12 at locus Glu D1. We made a study of Longmai 15 in detailS and found the biotype of Longmai 15 with HMW glutenin subunits 5+10 at locus Glu D1. We analysed the origin of the biotype of Longmai 15 with HMW glutenin subunits 5+10 and how to utilize at present breeding of high quality wheat.