60Co—r射线辐射番茄种子对根尖细胞遗传学效应的影响

Effects of 60 Co r Ray Radiation on Tomato′s Root Tip Cells With Radiating Tomato Seeds

用６０Ｃｏ－ｒ射线辐射番茄干种子，水培法生根后，观察根尖细胞有丝分裂中出现的桥、断片（含落后）、粘连、微核等染色体畸变类型，辐射剂量在５—１０ｋｒａｄ范围内畸变率较低；在１０—２０ｋｒａｄ范围内畸变率最高，畸变类型最丰富，在此范围内随剂量增加，断片数量增多，桥的类型除单桥外还出现多桥且数量增多，在２０ｋｒａｄ以上范围的高辐射剂量对染色体破坏力力较大，不利于番茄育种和生长。经对照实验和统计分析可知，辐射剂量的增加对有丝分裂有抑制作用。

We studied the effect of tomato root tip cells radiated tomato dry seeds with 60 Co-r Ray, after they grew root in the water, observed chromosome bridge, chromosome fragments (including lagging chromosome), adhesion, micronucles ect abnormal chromosome type. The result show that the abnormal rate is lowest, when irradiation dose is in range 5-10 Krad; it is the highest and the types of abnormal chromosome is the most abundant, when the irradialion dose is in 10-20 Krad. In this range, quantity of the chromosome fragment is increasing, when the irradiation dose is enhanced, poly-bridge exist, besides single chromosome bridge. Chromosome are damaged seriously, when the irradiation dose is over 20 Krad. This dose is harmful to growth of tomato and the cells. By C. K. experiment and statistics. We can say, the irradiation dose controls the splits of root tip's cells.