摘要
本实验对ＴＭＶ抗性和敏感的两个番茄品种ｃｔ－ＤＮＡ进行ＲＡＰＤ分析，结果发现在２０个引物中有４个引物的扩增产物存在明显差异，共显示出１４条差异带。这可能是由于ｃｔ－ＤＮＡ碱基顺序变异或小片段ＤＮＡ插入或缺失造成的，深入研究将对探讨番茄抗ＴＭＶ的分子机理，进而用遗传工程方法培育抗病品种具有重要意义。
In the present study, we analyse the ct-DNA of tomato which is resistant and sensitive to TMV with RAPD. We find difference in four of the twenty primers. 14 different bands are showed. We guess this maybe alternation of base sequence or insertion or deletion of short DNA sequence. It will play an important role in study of machinism of TMV resistance and construction of virus resistant tomato.
出处
《植物研究》
CAS
CSCD
北大核心
1996年第1期114-117,共4页
Bulletin of Botanical Research
基金
国家自然科学基金
