雨堡组织培养的研究

The Tissue Culture of Regensberg

取雨堡当年生的枝条上饱满的未萌发的侧芽为外植体，培养于ＭＳ培养基上，其中附加各种不同种类及浓度的激素。在附加６—ＢＡ１．０ｍｇ／ｌ的ＭＳ培养基上，诱导芽的效果最好；在附加６—ＢＡ１．０＋ＮＡＡ０．０５＋ＺＴ０．１ｍｇ／ｌ的ＭＳ培养基上，芽的增殖效果最佳；在附加ＫＴ０．３＋ＮＡＡ０．０５＋ＺＴ０．１ｍｇ／ｌ或６—ＢＡ０．３＋ＮＡＡ０．０５＋ＺＴ０．１ｍｇ／ｌ的ＭＳ培养基上，最有利于壮苗；在１／２ＭＳ培养基中，附加ＩＢＡ０．５＋ＮＡＡ０．３ｍｌ／ｌ或ＩＡＡ０．５＋ＮＡＡ０．３ｍｇ／ｌ的培养基中，生根效果最佳。试管苗高２—３ｃｍ，且具有４—８条短根时，开瓶煅炼３—５天，移入培养土中生长的效果好，种植在保温保湿环境中，试管苗成活率高。

The tissue culture of Regensberg with the tip of shoots as the explants was experimnented in MS medium with addition of different contentration and kinds of multiplication.The results show that sprouting of bud was best on MS medium with addition of 6-BA 1.0mg/l;and the multiplication of adventitious buds was best on MS medium with the addition of 6-BAl.o+ NAA0.05mg/l;and vigorous and healthy bud was best on MS mdeuim with addition of KT0.3+NAA 0.05+ZT0.1mg/lor6-BA0.3+NAA0.05+ZT0.1mg/l;and the growth of roots was best on 1/2 MS medium with the addition IBA0.5+NAA0.3mg/lor IAA0.5+NAA0.3mg/l.It was better to transplant from test-tube grew to 2-3 cm high and 4-8roots and after the plantlets stayed 3 or 5 days without cap of test-tube in the experimental. The plantlets had better percentage of survival when it had the situation with deeping degree of temperature and humidity.