小牛血去蛋白提取物取代小牛血清培养K562细胞的研究

The Study of Deproteined Extract from Calf Blood Substitute of Calf Serum on Leukemia Cell Line K562 in Vitro

目的 评价小牛血去蛋白提取物（DECB）取代小牛血清培养K562的生长情况,从而分析DECB对白血病细胞生长方面的意义及其可能的作用机制.方法应用形态学、MTT分析法、流式细胞术对在低血清以及无血清条件下的白血病细胞进行检测和观察.结果人白血病K562细胞在DECB取代小牛血清的培养基中,细胞发生一系列形态学改变,DECB在一定范围内（0.2%～1%）对K562的增殖率有剂量和时间的依赖关系.结论DECB在一定范围内能够代替小牛血清在细胞培养过程中起作用,有利于细胞的生长与增殖.

Objective To acess thc growth substitution of DECB institute calf serum on K562. Methods Cell morphology, MTr assay and flow cytometry were used to examine in leukemia K562 cell cultured in low concentration serum and free serum. Resuits Cell treated with DECB have much changes in morphology. The growth proliferation effect of DECB on K562 cells was time and dosage dependent. Conclusions DECB have a direct proliferated effect on K562 cell line.