人乳头瘤病毒16型E2/E6癌基因与宫颈癌关系的研究

Human pappilomavirus type 16 E2/E6 oncogene in cervical cancer

目的探讨人乳头瘤病毒16型(HPV16)E2/E6癌基因在宫颈癌发生中的作用。方法采用以医院为基础的1∶1配比病例对照研究,应用热启动PCR法检测经病理诊断确诊的74例宫颈鳞癌新发病例,及同期就诊的74例宫颈良性肿瘤患者的高危型HPV16 E2/E6癌基因的状况。结果病例组HPV16 E2、E6阳性率(41.89%,52.70%)高于对照组(17.57%,21.62%),差别有统计学意义,OR分别为3.250(1.471-7.178)和3.875(1.824-8.233);病例组HPV16 E2缺失率较对照组略高。结论宫颈癌的发生与HPV感染,特别是高危型HPV16的感染密切相关;HPV16 E2基因缺失、E6基因高阳性率在宫颈癌的发生发展中起重要作用。

Objective To explore the role of human papillomavirus type 16（HPV16） E2/E6 oncogene in cervical cancer. Methods This study was a hospital-based case-control study in Tumour Hospital of Shanxi province during 2001 to 2002. Seventy-four women with invasive cancer and controls with hysteromyoma were matched to cases in a 1 ： 1 ratio. The presence of HPV16 E2/E6 oncogenes was tested in cervical tissue using PCR. Results The expressions of HPV16 E2/E6 in patients（41.89%, 52.70% ）were significantly higher than that in controls（ 17.57 %, 21.62 % ）, OR were 3. 250 （ 1.471 - 7.178） and 3. 875 （ 1. 824 - 8. 233） ; absence of HPV16 E2 in patients was slightly higher than in controls. Conclusion The infection of HPVs, especially high-risk HPV16 is related to the development of cervical cancer;the absence of HPV16 E2 and the expression of HPV16 E6 is more significant to cervical cancer.