二至天癸颗粒提高卵细胞质量与小鼠卵巢内IGF-1R mRNA表达量关系的研究

Experimental Study on Effect of Er'zhi Tiangui Granule in Improving Quality of Oocyte and Its Correlation with Level of Insulin-like Growth Factor-1R mRNA Expression in Ovary of Mice

目的探讨二至天癸颗粒提高卵细胞质量的机理。方法90只小鼠分为6组,实验1组与对照1组观察排卵后优质卵数目。实验2组与对照2组观察鼠胚卵裂率。用原位杂交的方法对实验3组与对照3组鼠卵颗粒细胞IGF-1R mRNA表达量进行检验。结果实验组与对照组优质卵率分别为〔(78±8)%、(71±5)%〕,卵裂率分别为〔(88±3)%、(83±5)%〕及颗粒细胞IGF-1R mRNA表达量分别为〔(0·4890±0·0454)、(0·4439±0·0283)〕,两组比较差异有显著性(P<0·05)。优质卵率与颗粒细胞IGF-1R mRNA表达量呈正相关。结论二至天癸颗粒提高卵细胞质量的机理可能与提高颗粒细胞IGF-1R mRNA的表达量有关。

Objective To explore the mechanism of Er＇zhi Tiangui Granule （ETG） in improving the quality of oocyte. Methods Ninety mice were randomly divided into 6 groups, The number of high-quality oocytes was comparatively observed in the 1st experimental group and the 1st control group; the embryonic cleavage rate was observed in the 2nd experimental group and the 2nd control group and the quantity of insulin-like growth factor-lR mRNA （IGF-1R mRNA） expression in ovarian granular cells was determined by using in situ hybridization in the 3rd experimental group and the 3rd control group, Results The high-quality oocytes rate, the embryonic cleavage rate and the quantity of IGF-1R mRNA expression in the three paired groups was （78 ± 8）% vs （71±5）%, （88±3）% vs （83±5）%, 0.4890±0.0454 vs0.4439±0.0283, respectively. The difference between the experimental groups to the respective control groups was significant （all P 〈0.05）, and positive correlation was shown between the high-quality oocytes rate and the quantity of IGF-1R mRNA expression. Conclusion The mechanism of ETG in improving the quality of oocyte may be related with the elevation of IGF-1R mRNA level in ovarian granular cells.