摘要
目的:研究糖基化终产物(AGEs)对人肾小球系膜细胞半乳糖凝集素-3(galectin-3)表达的影响。方法:将培养的人肾小球系膜细胞(HRMC)用不同浓度(50、100、200及400 mg.L-1)的AGEs干预24 h或同一浓度(200 mg.L-1)的AGEs干预0、24、48及72 h,以无血清培养基(DMEM)和相应浓度的牛血清白蛋白(BSA)为对照。用RT-PCR检测细胞中半乳糖凝集素-3的mRNA水平。结果:不同浓度AGEs组HRMC的半乳糖凝集素-3 mRNA水平与对照组相比均有显著差异(P<0.01),且随着AGEs浓度的增高,半乳糖凝集素-3 mRNA水平也逐渐增高(P<0.05);AGEs干预HRMC 24、48、72 h,半乳糖凝集素-3mRNA水平与对照组相比有显著差异(P<0.05),且随着干预时间的延长,半乳糖凝集素-3 mRNA水平也逐渐增高(P<0.01)。结论:AGEs以时间及剂量依赖的方式促进HRMC半乳糖凝集素-3 mRNA的表达。
Objective To study the effect of advanced glycation end products(AGEs) on the expression of galecfin- 3 in cultured human renal mesangial cells(HRMC). Methods The AGEs-BSA was prepared by incubating bovine serum albumin(BSA) with glucose. The cultured HRMC were intervened with the same concentration of BSA and AGEs- BSA(50,100,200,400 mg · L^-1 ) for 24 h or 200 mg · L^-1 AGEs-BSA for 0,24,48 or 72 h respectively. The mRNA expression of galectin-3 in HRMC were analyzed by RT-PCR. Results Compared with that in cells incubated with DMEM and BSA,the level of galectin-3 mRNA in cells incubated with 50,100 or 200,400 mg · L^-1 AGEs-BSA respectively for 24 h ( P 〈 0.01 ) or with 200 mg· L ^-1 AGEs- BSA for 0,24,48 or 72 h respectively ( P 〈 0.05 ) increased significantly. Moreover, with the increased concentration of AGEs- BSA ( P 〈 0.05 ) or the expanded incubating time ( P 〈 0.01 ), the level of galectin-3 mRNA increased. Conclusion The AGEs- BSA can stimulate the expression of galectin- 3 in cultured HRMC in a concentration- and time-dependent manner.
出处
《东南大学学报(医学版)》
CAS
2006年第3期174-177,共4页
Journal of Southeast University(Medical Science Edition)
基金
江苏省教育厅高校自然科学研究项目(02KJA320001)
教育部科学技术研究重点项目(204051)