两种细胞培养流感病毒的滴度比较

Comparison of Influenza Virus Titers Cultured in Vero and MDCK Cells

目的探讨甲1(A1)型流感病毒在Vero细胞和MDCK细胞培养的可行性,确定最佳培养条件。方法将流感病毒接种于Vero细胞和MDCK细胞,在含有不同浓度胎牛血清或胰酶维持液中培养,于不同时间收获病毒液,检测血凝素滴度(HA)。结果胎牛血清对病毒的繁殖有明显抑制作用。加入适量胰酶(约5μg/ml)可提高病毒产量。最佳收获病毒时间为72～96h。在MDCK细胞上的病毒HA滴度高于Vero细胞。结论两种组织细胞培养流感病毒结果相近,Vero细胞和MDCK细胞均可用于培养流感病毒。

Objective To explore the feasibility of culture of influenza virus type A1 in Vero and MDCK cells and optimize the culture condition. Methods Inoculate influenza virus type A1 into Vero and MDCK cells and culture in media containing fetal bovine serum（FBS） or trypsin at different concentrations. Harvest the virus liquid at different time for determination of HA titers. Results FBS inhibited the proliferation of influenza virus significantly. The addition of about 5μg/ml of trypsin increased the virus yield. The optimal time for harvesting virus was 72-96 h after cultivation. The HA titer of influenza virus in MDCK cells was significantly higher than that in Vero cells. Conclusion Both Vero and MDCK cells were suitable for culture of influenza virus and development of influenza vaccine.