益康唑莪术油复方制剂抗炎作用的实验研究

Experimental study of Enconazoleezhuyou compound preparation

目的研究益康唑莪术油复方制剂(抗阴道炎药)抗炎作用。方法将♀大鼠随机分组,建立阴道白色念珠菌、大肠杆菌、金黄色葡萄球菌感染模型,分别给予双唑泰栓(阳性对照)、益康唑莪术油复方制剂3个剂量组,每天给药1次,连用7天;以阴道菌培养菌落数和阴道组织炎细胞浸润程度为观察指标。结果益康唑莪术油复方制剂对大鼠阴道感染白色念珠菌、大肠杆菌、金黄色葡萄球菌有明显治疗作用,2个剂量组(648,324mg·kg-1)和阳性对照药均与赋形剂对照组比较,均有显著性差异(P<0.05)。结论益康唑莪术油复方制剂可明显降低大鼠阴道感染白色念珠菌、大肠杆菌、金黄色葡萄球菌的数量,明显降低阴道组织炎细胞浸润程度,对阴道正常菌群无明显影响。

Objective To investigate anti - inflammatory effects of Enconazoleezhuyou compound preparation. Methods Famle rats were infected with Candida albicans, Escherichia. coli, S. aureus to establish vaginitis model. The vaginitis rats were randomized into groups treated with Shuangzuotai and Enconazoleezhuyou compound preparation. Enconazoleezhuyou compound preparation group were treated with three different dosages. The drug was vaginally administered dayly for 7 days. Resuits Enconazoleezhuyou compound preparation had significant therapeutic effect on infecting invaginitis of rats induced by Candida albicans, Escherichia. coli, S. aureus . Dose of 648 mg · kg^-1 and 324 mg· kg^-1 Enconazoleezhuyou compound preparation groups and positive contral Shuangzuotai group had significant difference compare to excipient control （P 〈 0.05, P 〈 0.01 ）. Conclusion Enconazoleezhuyou compound preparation has bactericidal activity against Candida albicans, Escherichia. coli, S. aureus . The number of these bacterials＇colonies was significantly reduced . Enconazoleezhuyou compound preparation can also attenuate the degree of inflammatory cells infiltration but has no effect on normal flora in vagina.