重组人干扰素γ体外抗呼吸道合胞病毒的作用观察

Effect of recombinant human interferon-γfor the inhibition of respiratory syncytial virus in vitro

目的:观察国产重组人干扰素γ(rHuIFN-γ)体外抗呼吸道合胞病毒(respiratory syncytial virus,RSV)的作用。方法:RSV在96孔板中通过Hela细胞进行增殖,不同浓度的IFN-γ加入孔板中,测定药物对RSV感染所致细胞病变的抑制作用。结果:IFN-γ在HeLa细胞上的半数中毒浓度(TC_(50))为2．80万IU·mL^(-1),在HeLa细胞中抗RSV的半数有效浓度(EC_(50))为4．78 IU·mL^(-1),治疗指数(TI)为5857．74。结论:IFN-γ在体外对RSV有显著抑制作用,并呈明显的量效关系。

Objective：To assess the effect of recombinant human interferon-γ for the inhibition of respiratory syncytial virus （RSV） in vitro. Methods： RSV was proliferated by HeLa cells. A series of concentrations of the recombinant human interferon-γ were incubated with the HeLa cells to monitor the inhibition of the rh interferon-γ to the cytopathogenic effect （CPE） of RSV. Results： TC50 of the rh interferon-γ in the HeLa cells was 2. 80 × 10^4 IU·mL^-1, EC50 was 4.78 IU·mL^-1 and TI was 5 857.74. Conclusion： The rh interferon-γ significantly antagonized the RSV pathogenesis in vitro in a dose-dependent setting.