摘要
目的:观察国产重组人干扰素γ(rHuIFN-γ)体外抗呼吸道合胞病毒(respiratory syncytial virus,RSV)的作用。方法:RSV在96孔板中通过Hela细胞进行增殖,不同浓度的IFN-γ加入孔板中,测定药物对RSV感染所致细胞病变的抑制作用。结果:IFN-γ在HeLa细胞上的半数中毒浓度(TC_(50))为2.80万IU·mL^(-1),在HeLa细胞中抗RSV的半数有效浓度(EC_(50))为4.78 IU·mL^(-1),治疗指数(TI)为5857.74。结论:IFN-γ在体外对RSV有显著抑制作用,并呈明显的量效关系。
Objective:To assess the effect of recombinant human interferon-γ for the inhibition of respiratory syncytial virus (RSV) in vitro. Methods: RSV was proliferated by HeLa cells. A series of concentrations of the recombinant human interferon-γ were incubated with the HeLa cells to monitor the inhibition of the rh interferon-γ to the cytopathogenic effect (CPE) of RSV. Results: TC50 of the rh interferon-γ in the HeLa cells was 2. 80 × 10^4 IU·mL^-1, EC50 was 4.78 IU·mL^-1 and TI was 5 857.74. Conclusion: The rh interferon-γ significantly antagonized the RSV pathogenesis in vitro in a dose-dependent setting.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2006年第9期691-694,共4页
Chinese Journal of New Drugs
关键词
重组人干扰素Γ
呼吸道合胞病毒
体外抗病毒
recombinant human interferon-γ
respiratory syncytial virus (RSV)
anti-virus in vitro