赤芍挥发性成分的GC-MS与直观框导式演进特征投影法分析

Analysis of Volatile Chemical Components in Radix Paeoniae Rubra by GC-MS and Heuristic Evolving Latent Projections Method

目的定性、定量分析赤芍中挥发性成分、方法利用气相色谱-质谱(GC-MS)进行检测,再通过直观推导式演进特征投影(HELP)法对产生的二维色谱-质谱数据进行解析,得到各组分的纯色谱曲线和质谱,根据其保留时间和质谱,在质谱库中进行相似检索,实现对组分的定性,再用总体积积分法进行定量。结果共分辨出57个色谱峰,通过质谱库对分辨的纯组分进行检索,鉴定了其中38个组分,占赤芍挥发成分总合量的95.21%。结论赤芍主要挥发成分为(Z,Z)-9,12-十八碳二烯酸,正十六碳酸和水杨醛。

OBJECTIVE To analyze the volatile chemical components of Radix Paeoniae Rubra（RPR）.METHODS Gas chromatography-mass spectrometry （GC-MS）, heuristic evolving latent projections （HELP） method, and overall volume integration methods were used.RESULTS Thirtyeight volatile chemical components in RPR were determined, accounting for 95.21% of volatile chemical components of RPR. CONCLUSION The mail volatile chemical components of RPR are （ Z, Z）-9,12-octadecadienoic acid, n-hexaclecanoic acid and 2-hydroxy-henzaldehyde.