摘要
目的通过神经干细胞(NSCs)移植治疗大鼠脑自由落体撞击伤,探讨NSCs对脑外伤功能恢复的影响。方法体外培养NSCs并用5-2溴脱氧尿嘧啶(B rdU)标记。自由落体撞击大鼠左侧大脑皮质运动感觉区以制作脑外伤模型,脑外伤后24h内移植NSCs,分别于脑外伤前、脑外伤后1、2、4周时行神经运动行为学评分(NMFE)和免疫组织化学染色检测B rdU、巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)和半乳糖脑苷酯(GalC)蛋白表达。结果大鼠自由落体撞击伤后,右下肢神经运动功能障碍明显。神经运动行为学评分在第1周时,实验组与对照组无明显差异,而在第2、4周时,实验组的评分明显低于对照组,差异有统计学意义(P<0.05)。第2、4周时实验组NSE、GFAP和GalC染色阳性细胞数多于对照组,nestin染色在第1周时表达最高,以后逐渐下降。B rdU阳性细胞在损伤灶中心区最高多,在损伤灶的远隔部位也有少许发现。结论NSCs移植有利于大鼠脑自由落体撞击伤后期的功能恢复。移植的NSCs可以在损伤部位存活并增殖分化与迁移。
Objective To investigate the effects of embryonic neural stem cells （NSCs） on the repair of rat brain weight-dropping injury. Methods NSCs were cultured in vitro ,labeled with BrdU , and transplanted into rat brain area injured by weight-dropping. The neurological motor function evaluation （NMFE） were used to test the functions at the 1, 2, 4 weeks post-injury. Immunohistochemistry was used to detect the expressions of BrdU , nestin ,NSE , GFAP and GalC. Results There was significant difference in NMFE between experiment group and control group（P 〈0.05） at 2,4 weeks. At 2, 4 week, there were more expressions of NSE, GFAP and GalC in experiment group than in control group. Expression of nestin was higher at 1 week than at 2, 4 week. BrdU positive cells were mainly detectable in traumatic area and simultaneously less at the distance from traumatic area using immunohistochemical staining. Conclusion NSCs have effects on rat brain weight-dropping injury. Transplanted NSCs can survive, proliferate, differentiate and immigrate in traumatic area.
出处
《现代实用医学》
2006年第3期148-151,共4页
Modern Practical Medicine
