大头芋的组织培养

Tissue Culture of Amorphophallus dunnii Tutcher

以海南野生大头芋(AmorphophallusdunniiTutcher,也称南蛇棒)的叶柄、主叶脉为外植体,筛选适宜各培养阶段的培养基。结果表明:以叶柄、主叶脉为外植体进行愈伤组织诱导,诱导率和分化率均可达100%,且增殖系数较高;MS+6-BA4.0mg/L+NAA0.1mg/L+蔗糖30g/L培养基适宜愈伤组织诱导及继代增殖培养;MS+6-BA4.0mg/L+NAA0.01mg/L+蔗糖30g/L培养基适宜不定芽诱导及增殖培养;MS+NAA0.2mg/L+蔗糖30g/L培养基适宜壮苗及生根培养。

Media for tissue culture ofAmorphophallus dunnii Tutcher with the petiole and midrib as the explants were screened. The results showed that the inducing and differentiation rates of the explants were upto 100% on the media screened, and that the propagation coefficient was very high. The optimum media found at each culture stages were： MS ＋ 6-BA 4.0 mg/L ＋ NAA 0.1 mg/L ＋ sucrose 3% for formation of callus and multiplication; MS ＋ 6-BA 4.0 mg/L ＋ NAA 0.01 mg/L ＋ sucrose 3% for formation of adventitious buds and multiplication, and MS ＋ NAA 0.2 mg/L ＋ sucrose 3% for robust growth of the seedlings and for rooting.