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大豆GmLls1基因的克隆及表达调控分析 被引量：3

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摘要 玉米的叶片坏死斑点(lethal leaf-spot 1,Lls1)基因及其拟南芥中的同功能基因已证明编码叶绿素分解代谢中的关键酶脱镁叶绿酸氧化酶(pheide a oxygenase,PaO)．为了深入研究大豆叶片衰老过程中叶绿素分解代谢的调控机制,从大豆中克隆了玉米Lls1的同源基因,其cDNA全长1817bp,命名为GmLls1(GenBank登录号：DQ154009)．序列分析表明,该基因与拟南芥、玉米、豇豆和番茄等的Lls1及其同功能基因具有很高的同源性,其编码蛋白含有典型的Rieske[2Fe-2S]结构域、非亚铁血红素铁结合域和C-末端CXXC基序,这些都是PaO功能蛋白所具有的典型结构特征．RT-PCR结果显示,在大豆叶片自然衰老、人工黑暗诱导衰老和子叶衰老3个系统中,GmLls1基因都表现出明显的衰老上调趋势．进一步分析发现,在因敲减类受体蛋白激酶rlpk2基因而导致衰老延缓的大豆转基因叶片中,GmLls1的表达显著下降,而在因过表达rlpk2而导致加速衰老的转基因叶片中,GmLls1的转录本积累明显增加,暗示RLPK2介导的信号途径可能参与调控GmLls1在大豆叶片衰老过程中的表达,而rlpk2-RNAi转基因离体叶片光照下表现出lls1突变体典型的斑状失绿坏死表型则进一步支持了上述推论．
作者 李鹏丽 马媛媛 李小平 张丽文 王勇 王宁宁
机构地区 南开大学生命科学学院植物生物学与生态学系
出处 《科学通报》 EI CAS CSCD 北大核心 2006年第9期1034-1041,共8页 Chinese Science Bulletin
基金 国家自然科学基金资助项目(批准号:30270140).
关键词 大豆 叶片衰老 脱镁叶绿酸氧化酶 叶片坏死斑点基因 类受体蛋白激酶
分类号 S565.1 [农业科学—作物学]
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参考文献32

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共引文献19

同被引文献47

  • 1马媛媛,甘睿,王宁宁.植物富含亮氨酸重复序列型类受体蛋白激酶的生物学功能[J].植物生理与分子生物学学报,2005,31(4):331-339. 被引量：29
  • 2Pru·inska A et al. Plant Cell, 2007, 19(1): 369-387
  • 3Harpaz-Saad Set al. Plant Cell, 2007, 19(3): 1007-1022
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  • 10Pru·inska A et al. Plant Physiol, 2005, 139(1): 52-63

引证文献3

二级引证文献23

科学通报
2006年 第9期

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