氯化镧诱导脑胶质瘤SHG44细胞凋亡作用的机制

Experiment research of apoptotic effects on human brain glioma SHG44 with LaCl_3

目的探讨氯化镧对人脑胶质瘤细胞株SHG 44细胞诱导凋亡及其作用机制。方法体外培养人胶质瘤细胞,流式细胞仪、电镜检查细胞周期和超微结构的改变,原位杂交技术及免疫组化染色技术检测bcl-2、bax基因、蛋白表达情况。结果氯化镧能促进入脑胶质瘤SHG 44细胞发生凋亡,明显增加G1期DNA含量,减少S期DNA含量。使bax基因、蛋白表达显著增强,bcl-2、bax基因蛋白表达显著减弱(P<0.05)。结论氯化镧可抑制人脑胶质瘤SHG 44细胞的生长,并促进其凋亡。

Objective： To investigate the feasibility and mechanism of LaCl3 induced apoptosis in human glioma SHG44. Methods： The human glioma SHG44 cells were cultured in vitro,the cell circle and ultrastructure were detected by flow cytometry and electron microscope. The protein expression and translation of bax,bcl-2 were detected by immunohistochemistry and in situ hybridization methods. Results ： The LaCl3 could inhibit gene the growth of SHG44 and induce apoptosis. The DNA content of SHG44 increased in G1 cycle and decreased in S cycle obviously. Typical apoptosis cells appeared. Both of protein expression and mRNA translation of bcl-2 gene decreased, the bax gene protein expression and mRNA translation increased（ P 〈 0. 05）. Conclusion： LaCl3 can inhibit the proliferation of SHG44 cells in vitro and cause apoptosis.