摘要
采用组织块培养法培养的人胚胎肝上皮细胞,于培养后第3～9天即在贴壁组织块周围长出,并可在体外较长期进行培养;传至第8代时仍能分泌甲胎蛋白(AFP),含量为15～40ng/ml。组织块培养法不失为一种较为简单、实用、经济和有效的方法,为探索胚胎细胞体外培养方法提供了可供参考、借鉴的途径、本文还认为肝上皮样新生细胞长出与组织块贴壁率密切相关,并对影响组织块贴壁率的有关因素进行了讨论。
Growth of human embryonic liver cells could be seen around the tissue fragment culture adhering to the wall of test tube 3~9 days after incubation . The cells could be cultured for a long period of time in vitro. In 8th generation of. the cells they could still secret AFP.the concentration reached to 15~40 ng/ml. The tissue fragment culture is a simple、practical、e-conomical and effective method. The study supplied an effective way to explor the culture of human embryonic liver cells in vitro. It was also suggested that the growth of human embryonic liver cells correlated invesely with the rate of tissue fragment adhering to the wall of test tube. Some factors affecting the rate of tissue fragment adhering to the wall of test tube were discussed.
