天牛科纤维素酶同工酶研究

STUDY ON ISOZYMES OF C_x-CELLULASE IN CERAMBYCIDAE

用聚丙烯酰胺凝胶等电聚焦电泳方法分析了天牛科两个亚科１８种雌成虫、１２种幼虫消化道中肠组织纤维素酶复合酶中Ｃｘ酶同工酶．Ｃｘ酶同工酶在亚科间、族间差异较大；同族内各属间的酶谱差异也较大；同属内不同种间酶谱较接近，但每种各具其特征酶带，彼此易区分。Ｃｘ酶同工酶用于亲缘关系相近种的分类、鉴定有重要价值，可作为天牛科分子分类的一个有用的辅助手段。文章还就楔天牛族Ｓａｐｅｒｄｉｎｉ的分类系统问题进行了讨沦．

The isozymes of Cx-Cellulase from the alimentary canals of 18 species of female adults,12 species of larvae of longicorn beetles representing two sub-families(Lamiinae, Cerambycidae)were investigated with isoelectricfocusing polyacrylamide gel electrophoresis method.Difference of isoenzymes among subfamilies and tribes are more conspicuous than that among genera,there are no any similar zymogram bands possessed by subfamilies or by tribes,Certain zymogram bands possessed in common by some genera,and also among the different species of the same genus,and there are usually some specificbands existed in different species.Moreover,according to the degree of diversity or similarity of Cx-Cellulase isozymes,it would be morereasonable to restore the genera Linda,Oberea and Phytoecia into tribe Phytoecini,and the genus Paraglenca into tribe Gleneini rather than to treat them as a single tribe Saperdini as in Breuning'Revision.