银杏叶提取物对大鼠肝星状细胞细胞因子和胶原合成及细胞增殖的影响

Effect of ginko biloba extract on the expression of cytokines and collagen and cell proliferation in rat hepatic stellate cells

目的研究银杏叶提取物抗肝纤维化的可能机理。方法用不同浓度(0,1,10,100,500μg·ml-1)的银杏叶提取物处理HSC-T6细胞24h和48h,采用RT-PCR法检测各组细胞转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)、Ⅰ型胶原、Ⅲ型胶原mRNA的表达;应用流式细胞仪检测细胞增殖周期的变化。结果银杏叶提取物在10,100,500μg·ml-1浓度能明显抑制TGF-β1、CTGF、Ⅰ型胶原、Ⅲ型胶原mRNA的表达(P<0.01或P<0.05),在一定范围内呈剂量和时间依赖性,影响HSC-T6的细胞周期,降低其增殖活性。结论银杏叶提取物可明显抑制HSC-T6细胞的增殖,抑制细胞因子及细胞外基质成分基因的表达,由此发挥抗肝纤维化的作用。

Objective To investigate the effect of ginko biloba extract （GbE） on the expression of cytokines and collagen and cell proliferation in HSC-T6 cell line Methods HSC-T6 cells were cultured with GbE at different dosages （1,10,100,500μg·ml^-1 ） and without GbE as control. The expression of transforming growth factor β1 （TGF-β1） , connective tissue growth factor （CTGF）, type Ⅰ and type Ⅲ collagen mRNA were determined by reverse transcription-polymerase chain reaction （RT-PCR） after 24 hour and 48 hour culture.The cell cycles were analyzed by flow cytometry. Resets GbE down-regulated the expression of TGF-β1 ,CTGF,type Ⅰ and type Ⅲ collagen mRNA in HSC-T6 cells （P〈0. 01 or P〈0. 05）. The effect was on a dose and time dependent manner. And it inhibited the proliferation of HSC-T6 cells Conclusions GbE could exert the inhibitory effect on HSC- T6 cells by down-regulating the expression of TGF-β1 and CTGF.