摘要
采用MTT和SRB法研究了双核铂(Ⅱ)配合物[{Pt(H2O)2I}-μOOC-COO-{Pt(H2O)2I}](BPI)对人体癌细胞的增殖抑制作用,又通过流式细胞法、Giemsa染色法、等离子体质谱法研究了它的抗癌机制.实验结果表明:在10μmol.L-1浓度下,该配合物对HL-60,BGC-823,Bel-7402,KB,Hela,HCT-8,MCF-7和EJ 8种肿瘤细胞都表现出有较高的活性,对HL-60、BGC-823和Bel-7402 3种肿瘤细胞的抑制率都在70%以上,它能阻止HL-60细胞G2+M→G1期的进行;对HL-60细胞的凋亡诱导作用不明显;在相同浓度情况下其与HL-60细胞的DNA键合量大于顺铂.
The antitumor activity of binuclcar platinum（Ⅱ） complex [{Pt（H2O）2I} μ-OOC-COO-{Pt（H2O）2I}] Was determined by MTT and SRB methods in vitro. At the same time, the anticaneer mechanism was studied by flow cytometry, Giemsa and Icp-Ms. The experimental results indicated that the complex had high activity against HL-60, BGC-823,Bel-7402,KB, Hela, HCT-8, MCF7 and EJ, and the inhibition rate was greater than 70 % against HL-60, BC-C-823 and Bel-7402 at concentration of 10μmol·L^-1 The cell cycle was arrested on the G2＋ M→G1 , the effect of the complex on apoptosis was not obvious, and the level of total platinum bound to DNA was higher than that of cisplatin under the same conditions.
出处
《浙江大学学报（理学版）》
CAS
CSCD
北大核心
2006年第3期304-308,共5页
Journal of Zhejiang University（Science Edition）
基金
浙江省自然科学基金资助项目(298068)
北京大学医学部天然药物及仿生药物国家重点实验室资助项目
关键词
双核铂配合物
抗肿瘤活性
细胞周期
凋亡
DNA
binuclear platinum complexes
antiumor activity
cell eycle
apoptosis
DNA
