外源蛋白在大肠杆菌中的可溶性表达策略

Strategies for Expression of Soluble Heterologous Proteins in Escherichia coli

长期以来,大肠杆菌一直是表达外源蛋白的首选表达系统.但由于外源蛋白在表达过程中容易被宿主细胞蛋白酶降解或者形成包涵体,其应用受到了限制.本文综述了在大肠杆菌中表达可溶外源蛋白的策略和进展,以期提高具有生物活性的外源基因的表达水平.

Escherichia coli has long been the primary expression system for the synthesis of heterologous proteins, but its utility is limited because many heterologous proteins are either prone to be degraded by the cellular proteases or to accumulate in insoluble form, typically as inclusion bodies. This paper reviews recent advances in the strategies for expression soluble heterologous proteins in Escherichia coli so as to improve the recovery yield of heterologous gene products in a biologically active form.