基于ICCD的快速荧光显微成像技术及在活细胞研究中的初步应用

ICCD-Based Fast Fluorescence Micro-Imaging Technique and Preliminary Application in Living Cells

利用像增强型CCD、氩离子激光器和氙灯等建立了一套快速荧光显微成像系统,并初步应用于活细胞研究。实时观测和拍摄了大鼠脑微血管内皮细胞增殖分裂过程中细胞内钙敏感荧光探针Fluo-3标记的钙离子浓度分布快速变化的图像,并选取动态图像中四个典型的点给出荧光强度灰度值的变化曲线。该系统可用于高灵敏实时记录活细胞内基于荧光显微成像的快速变化过程,为活细胞的研究提供了一种很好的观察和分析手段。

A fast fluorescence micro-imaging system using mainly intensified charge couple device（ICCD）, argon-ion laser, and xenon lamp was set up, and its preliminary application in living cells was presented. Real-time observation and imaging of fast concentration and distribution changing of intracellular Ca^2＋ labeled by Fluo-3, a fluorescent Ca^2＋ indicator, in the proliferation process of rat cerebral micro-vessels endothelial cells（rCMECs） were carried out, and curves of artificial intensity versus imaging sequence of four typical points were obtained. It is shown that the ICCD-based fast fluorescence micro-imaging system is a powerful tool for recording the real-time fast processes in living cells.