反射光谱装置及定量分析设计

The Reflection Spectrometer and Quantitative Analysis Design

设计了一种反射光谱测量装置.该装置由光纤传导并用于定量分析.通过测定还原糖与斐林试剂反应生成的Cu2O,建立测定还原糖的反射光谱分析方法.还原反应在微型反应池中进行,生成鲜红色Cu2O,光纤探头直接测定降于池底的沉淀.探讨了该方法的基本原理、影响因素及测定条件.该方法具有操作方便,灵敏度高,试剂用量少等优点.还原糖在20～120μg范围内呈良好的线性关系,用于实际样品测定,结果满意.

The reflection spectrometer with optical fibre transmission was designed and used in quantitative analysis. The reflection spectrometry was applied to the determination of reducing sugar in samples based on the reduction reaction of the reducing sugar with Fehling＇s reagent. The reduction reaction was carried out in a micro-reaction cell, and the reddish colored Cu2O produced was settled at the bottom of the reaction cell. The reflectance R∞, which is directly proportional to the amount of Cu2O produced, was measured by the reflection spectrometer. The fundamental principle, effect factors, and experimental conditions of the method were discussed. Linear relationship was obtained in the range of 20-120 μg of reducing sugar. The special features of this method were its simplicity in operation, relatively high sensitivity of determination, and the use of small amount of reagent. Satisfactory results were obtained in its application to the analysis of wine and honey samples.