里氏木霉LW1产纤维素酶的酶学性质

Enzymatic Characteristics of Cellulase Produced by Trichoderma Reesei LW1

研究了里氏木霉LW1所产纤维素酶的主要酶学性质。结果表明,该纤维素酶的最适温度为50℃,最适pH值为4.8;在30～50℃,pH 4.0～6.0范围内有较高的稳定性;90℃处理15 min,酶粉的CMC酶活和FPA酶活保存率分别为38.66%和52.68%;Ca2+、K+、Na+、Mg2+离子对酶活有激活作用,Mn2+、Zn2+、Cu2+离子有抑制作用。

Enzymatic characteristics of the crude enzyme from Trichoderma Reesei LW1 were studied. The optimal conditions for enzymatic reaction were 50℃, and pH value were 4.8; showed a high stability at temperature 30-50℃, pH 4.0-6.0; stayed 15 rain at 90℃ ,CMCase could be held 38.66%, and FPase can be held 52.68%; Ca^2＋, K^＋, Na^＋, Mg^2＋ were the activators and Mn^2＋, Zn^2＋, Cu^2＋ were the inhibitors of the enzyme.