摘要
研究了里氏木霉LW1所产纤维素酶的主要酶学性质。结果表明,该纤维素酶的最适温度为50℃,最适pH值为4.8;在30~50℃,pH 4.0~6.0范围内有较高的稳定性;90℃处理15 min,酶粉的CMC酶活和FPA酶活保存率分别为38.66%和52.68%;Ca2+、K+、Na+、Mg2+离子对酶活有激活作用,Mn2+、Zn2+、Cu2+离子有抑制作用。
Enzymatic characteristics of the crude enzyme from Trichoderma Reesei LW1 were studied. The optimal conditions for enzymatic reaction were 50℃, and pH value were 4.8; showed a high stability at temperature 30-50℃, pH 4.0-6.0; stayed 15 rain at 90℃ ,CMCase could be held 38.66%, and FPase can be held 52.68%; Ca^2+, K^+, Na^+, Mg^2+ were the activators and Mn^2+, Zn^2+, Cu^2+ were the inhibitors of the enzyme.
出处
《纤维素科学与技术》
CAS
CSCD
2006年第2期46-49,共4页
Journal of Cellulose Science and Technology
关键词
里氏木霉
纤维素酶
酶学性质
Trichoderma Reesei
cellulase
enzymatic characteristics