逆向法确定色氨酸对映体竞争型吸附等温线

Determination of competitive adsorption isotherms of tryptophan enantiomers by inverse method

Competitive adsorption isotherm data for two enantiomers of tryptophan, in which the stationary phase was silica-immobilized bovine serum albumin(BSA) and the mobile phase was a phosphate buffer,were measured by using the inverse method.The competitive bi-Langmuir isotherm model(Fisher coefficient is 1769.07, R2=0.9997) and equilibrium-dispersive model were selected by comparison of the experimental results and the interaction mechanism.The corresponding parameters were obtained by simulating the chromatogram of racemic mixture at 2×10 -4 mol·L -1 and 10 -3 mol·L -1.The results showed good agreement between the isotherm determined by the inverse method and the adsorption data (Fisher coefficient is 119.32, R2=0.9973) which confirmed the validity of the model selected.

Competitive adsorption isotherm data for stationary phase was silica-immobilized bovine serum phosphate buffer, were measured by using the inverse two enantiomers of tryptophan, in which the albumin （BSA） and the mobile phase was a method. The competitive bi-Langmuir isotherm model （Fisher coefficient is 1769.07, R^2= 0. 9997） and equilibrium-dispersive model were selected by comparison of the experimental results and the interaction mechanism. The corresponding parameters were obtained by simulating the chromatogram of racemic mixture at 2 × 10^-4 mol·L^-1 and 10.3 mol·L^-1. The results showed good agreement between the isotherm determined by the inverse method and the adsorption data （Fisher coefficient is 119.32, R^2 =0. 9973） which confirmed the validity of the model selected.