鹅膏蕈毒性与氧化损伤的实验研究

An experimental study on toxicity and oxidative damage induced by phalloides

目的探讨鹕膏蕈毒性与氧化损伤的定量敏感指标。方法将50只SD大鼠随机分5组，雌雄各半。腹腔注射染毒。根据动物实验的LD50（1．5～2．0mg／kg），分别按体重设0．5、1．0、1．5μg／kg 3个鹅膏蕈毒素染毒组和空白对照组及溶剂对照组。测定血液生化酶、脏器系数和氧化损伤指标。结果与空白对照组及与溶剂对照组比较，中高剂量组丙氨酸转氨酶（ALT）、超氧化物歧化酶（SOD）活力与丙二醛（MDA）含量明显增加（P〈0．05），高剂量组动物肝脏脏器系数明显增大（P〈0，05），心肌损伤特异性标志物天冬氨酸转氨酶（AST）活力水平变化明显（P〈0．05）。肝、肾中活力氧族含量未见明显差异（P均〉0．05）。结论血清ALT、AST、SOD活力、MDA含量，可作为评价鹕膏蕈毒素毒性与氧化损伤的定量敏感指标。

Objective To explore the toxicity and sensitive indexes of oxidative damage induced by phalloides in rats. Methods 50 SD rats were randomly divided into 5 groups, half males and half females. According to the result of acute toxicity, the rats were treated with phaUoides intraperitoneaUy at the doses of 0, 0（ solvent）, 0. 5, 1.0 and 1.5 μg/kg. Biochemical enzymes organ ratios, and indexes of oxidative damage were determined. Results Compared to the negative and solvent controis, the activity level of serum alanine aminotrasferase （ ALT）, the content of malondialdehyde （MDA） and superoxide dismutase（SOD） in the high and median dose groups were significantly increased （P 〈0. 05 ）. In the high dose group, organ ratio of liver, as well as the sign of damage to cardiac muscle （ aspartic-amino trasferase, AST） were significantly increased （ P 〈 0. 05）. Conclusion Serum ALT and AST, SOD and MDA are sensitive indexes for evaluating toxicity and oxidative damage induced by phalloides.