用仿生自组装法制备的新型生物复合材料及表征

Preparation and Characterization of a Novel Biologic Composite via Biomimetic Self-Assembly Method

通过仿生自组装法在室温下制备了纳米碳酸化羟基磷灰石/胶原（nCHAC）复合材料,并采用X射线衍射（XRD）、热分析（TGA）和透射电镜（TEM）进行了观察和分析.结果表明,该材料具有与天然骨相同的纳米尺度和胶原蛋白无机相,含有（2.8～14.7）w%的碳酸化HA成分;其显微结构是矿化的胶原纤维束,类似于天然骨的分级结构.揭示了碳酸化HA和胶原含量不同的复合材料中,具有微小差别的自组装单元.碳酸的百分含量影响矿物的晶体尺寸和胶原纤维的组装.因此,nCHAC复合材料是有前途的硬组织修复用材料,其所具有的特殊性能要归功于生物仿生制备出的nCHAC材料有与天然骨类似的成分和类似的微观结构.

Nano-carbonated hydroxyapatite/collagen（nCHAC）composite was prepared at room temperature via biomimetic self-assembly method.X-ray diffraction （ XRD ）, Thermogravimetric Analysis （ TGA ）, and Transmission Electron Microscopy （TEM）were performed. This composite shows the same inorganic phase of natural bone with nano-sized level and low crystallinity degree,and contains 2.8- 14.7 w% of carbonated content.TEM results confirm that the mierostructure of this composite is the mineralized collagen fiber bundle like the hierarchical structure of natural bone. The diameter of single mineralized collagen fiber is about 4 nm.Moreover, the little different assembly units of the composite with different carbonated and collagen were demonstrated.The carbonated percentage affects the mineral crystal size and collagen fibrillar assembly.Due to the biomimetic component and microstructure,the nCHAC composite is promising for hard tissue therapy.