麝香保心丸的小鼠含药血清促鸡胚尿囊膜血管生长的研究

Study on medicated serum containning Shexiang bao xin wan in promoting angiogenic effect of chick embryo chorioal-lantoic membrane

目的探讨麝香保心丸含药血清促鸡胚尿囊膜(CAM)血管生长的适当作用条件,为血清药理学筛选平台打下基础。方法以CAM为模型,观察小鼠不同喂药时间、喂药剂量和采血时间制备的麝香保心丸含药血清对CAM的促血管生长作用。结果麝香保心丸含药血清组的CAM血管生长数目明显多于空白对照组 (P<0．05),麝香保心丸含药血清组与血清灭活组的差异无显著性(P>0．05)。在临床等效剂量5倍剂量时喂药 4 d,末次喂药30 min后取得的麝香保心丸血清作用于CAM,其血管生长数目最多。结论用临床等效剂量的5 倍剂量喂养小鼠、喂药4 d、末次喂药后30 min得到的血清研究麝香保心丸的促血管生长作用效果最好。

Objective To appraise the most appropriate condition of angiogenic effect of Shexiang bao xin wan by using medicated serum. Methods Chorioallantoic membranes （CAM） of chick embryos were used as models, in a 37℃, 5%CO2 incubator, which were divided into groups of representatives for Shexiang bao xin wan sera made under different conditions and the control group whose sera were obtained from animals without drug feeding. The conditions undertaken included mice feeding days, feeding dosage of the drug and the exsanguinate time after the feeding. Vessel number was used as quantitation of the angiogenic response. Representative responses were studied on day 7 after fixation in methanol and acetone （ratiol ： 2） for half an hour. Results The CAM vessel number of Shexiang bao xin wan serum group was greater than that of the control group. The most obvious angiogenic response was observed after 4 feeding days, 5 times the animal feeding dosage （as clinical equivalent） and 30 min after feeding. No obvious angiogenic response was observed when the sera were inactivated. Conclusions The best study condition of Shexiang bao xin wan serum was at 4 mice feeding days, 5 times mice feeding dosage （as clinical equivalent） and 30 min after feeding as the exsanguinate time.