摘要
利用国产原材料对国外的ＴＥＳＴ卵黄缓冲液进行改良，获得ＨＴ卵黄缓冲液。用该缓冲液保存１５例有生育力男性精子，并做了新鲜精子与２４小时、４８小时、７２小时复苏后精子的ＳＰＡ、存活率和活动度对照观察。发现精子复苏后存活率明显下降，活动度也明显下降，Ｐ＜０．０５。但穿透率却出现上升趋势，Ｐ＜０．０５。结果表明∶ＨＴ卵黄液可在４℃～８℃中短期保存精子，并且使复苏后的具有活力的精子体外获能能力加大，导致ＳＰＡ率明显上升。作者认为ＨＴ卵黄液为精子染色体的制备在临床应用奠定了一些基础。
ESTyolkbuferwaschangedwithHepes,sodiumchloride,andTris,whichweremadeinChina.AnewkindofHTyolkbuferwasestablished.Thespermsfrom15maleswhohavenormalfertilitywerestoragedinthe4℃～8℃HTyolkbufer.After24,48,72hours,thespermswereresuscitedin37℃.TheratesofSPA,survivyandviabilitywereob-served,andcomparedwiththatofspermsfromfreshsemen.Therearestatisticaldiferentesbetweenthetwogroups(P<0.05),therateofSPAinstoragegroupincreased,theotherratesofsurvivyandviabilitydecreased.TheresultstoldusthatwecanusetheHTyolkbufertostoragethespermat4℃～8℃inashorttermtoincreasetherateofSPAandpreparingthehumanhaploidchromosomessharingonesemenatdiferenttime.
基金
国家自然科学基金
四川省科委资助
